Simultaneous Quantification of Avermectins in Six Aquatic Foods by UHPLC/FLD with Precolumn Derivatization

In this study, a fast, concise and reliable ultra-high performance liquid chromatography-fluorescence (UHPLC/FLD) detection method for simultaneous quantification of avermectins (AVMs), including avermectin (AVM), ivermectin (IVM), emamectin (EMM), moxidectin (MOX) doramectin (DOR) in six aquatic foods was established. Based on the QuEChERS pretreatment method, samples were extracted with 0.2% (v/v) ammonia acetonitrile. N-methyl imidazole mixed acetonitrile (1:1, v/v) trifluoroacetic anhydride (1:2, used as derivatization reagents. The mobile phase consists water flow rate 1.0 mL/min. An Infinity Lab Poroshell 120 EC-C18 column optimum chromatographic separation target analytes at 40 °C; excitation emission wavelengths set 365 nm 465 nm, respectively. kinds foods, limits (LODs) AVM, IVM, EMM, MOX, DOR 2.7 μg/kg, 1.8 2.1 1.2 respectively, (LOQs) 5 4.5 3.5 μg/kg 5.0 recoveries all above 85.38% when spiked compounds concentration level 5, 10, 50, 100 μg/kg. intra-day inter-day relative standard deviations (RSDs) less than 15%. This considers requirements sensitivity, accuracy, economics instrument.